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Yeast Extract Agar

[所属分类:培养基配方] [发布时间:2021-10-18] [发布人:网站管理员2] [阅读次数:] [返回]

Yeast Extract Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

Yeast extract.................................................................................. 1.0g

Buffer solution ...........................................................................2.0mL

pH 6.0 ± 0.2 at 25°C

Buffer Solution:

Composition per 400.0mL:

KH2PO4....................................................................................... 60.0g

Na2HPO4 ..................................................................................... 40.0g

Preparation of Buffer Solution: Add 40.0g of Na2HPO4 to

300.0mL of distilled/deionized water. Mix thoroughly. Add 60.0g of

KH2PO4. Mix thoroughly. Adjust pH to 6.0.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min

at 15 psi pressure–121°C. Pour into sterile Petri dishes.

Use: For the identification of Histoplasma capsulatum, Blastomyces

dermatitidis, and Coccidioides immitis.