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Yeast Extract Rose Bengal HiVeg Broth Base with Sorbose

[所属分类:培养基配方] [发布时间:2021-10-18] [发布人:网站管理员2] [阅读次数:] [返回]

Yeast Extract Rose Bengal HiVeg Broth Base

with Sorbose

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Na2HPO4 ................................................................................... 17.25g

Yeast extract.................................................................................. 5.0g

Synthetic detergent........................................................................ 2.0g

NaCl .............................................................................................. 1.0g

Sodium pyruvate ........................................................................... 1.0g

Rose Bengal ................................................................................ 0.04g

MgSO4 ........................................................................................ 0.01g

Sorbose solution.....................................................................100.0mL

pH 7.9 ± 0.2 at 25°C

Source: This medium, without sorbose solution, is available as a pre

mixed powder from HiMedia.

Sorbose Solution:

Composition per 100.0mL:

Sorbose.......................................................................................... 4.0g

Preparation of Sorbose Solution: Add sorbose to distilled/deion

ized water and bring volume to 100.0mL. Filter sterilize.

Preparation of Medium: Add components, except sorbose solu

tion, to distilled/deionized water and bring volume to 900.0mL. Mix

thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 45–-50°C. Aseptically add 100.0mL of

sterile sorbose solution. Mix thoroughly. Pour into sterile Petri dishes

or distribute into sterile tubes.

Use: For the cold enrichment for recovery of Yersinia enterocolitica

and Yersinia pseudotuberculosis from foods.