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Yeast Fermentation HiVeg Broth Base with Carbohydrate (Bromcresol Purple HiVeg Broth Base)

[所属分类:培养基配方] [发布时间:2021-10-20] [发布人:网站管理员2] [阅读次数:] [返回]

Yeast Fermentation HiVeg Broth Base

with Carbohydrate

(Bromcresol Purple HiVeg Broth Base)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Plant peptone............................................................................... 10.0g

NaCl .............................................................................................. 5.0g

Plant extract .................................................................................. 3.0g

Bromcresol Purple ...................................................................... 0.04g

Carbohydrate solution..............................................................50.0mL

pH 7.0 ± 0.2 at 25°C

Source: This medium, without carbohydrate solution, is available as

a premixed powder from HiMedia.

Carbohydrate Solution:

Composition per 100.0mL:

Carbohydrate............................................................................... 10.0g

Preparation of Carbohydrate Solution: Add carbohydrate to

distilled/deionized water and bring volume to 100.0mL. Adonitol, ara

binose, cellobiose, glucose, dulcitol, fructose, galactose, inositol, lac

tose, maltose, mannitol, raffinose, rhamnose, salicin, sorbitol, sucrose,

trehalose, xylose, or other carbohydrates may be used. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, except carbohydrate

solution, to distilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Distribute in 10.0mL vol

umes into test tubes containing inverted Durham tubes. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 25°C. Add 0.5mL of sterile

carbohydrate solution to each tube.

Use: For the determination of carbohydrate fermentation reactions of

microorganisms.