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Yeast Malate Medium (LMG 176)

[所属分类:培养基配方] [发布时间:2021-10-20] [发布人:网站管理员2] [阅读次数:] [返回]

Yeast Malate Medium

(LMG 176)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1001.0mL:

KH2PO4·12H2O ............................................................................ 1.0g

NaHCO3........................................................................................ 1.0g

Sodium malate .............................................................................. 1.0g

Yeast extract.................................................................................. 1.0g

(NH4)2SO4 .................................................................................... 0.5g

Trace elements solution .............................................................1.0mL

pH 6.8–7.0 at 25°C

Trace Elements Solution:

Composition per liter:

H3BO3 ........................................................................................... 0.3g

CoCl2·6H2O .................................................................................. 0.2g

ZnSO4·7H2O ................................................................................. 0.1g

MnCl2·4H2O................................................................................ 0.03g

Na2MoO4·2H2O .......................................................................... 0.03g

NiCl2·6H2O ................................................................................. 0.02g

CuCl2·2H2O ................................................................................ 0.01g

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.8–7.0.

Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–

121°C.

Use: For the cultivation of Rhodopseudomonas viridis.