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Yeast Malt Extract Agar (YM Agar)

[所属分类:培养基配方] [发布时间:2021-10-20] [发布人:网站管理员2] [阅读次数:] [返回]

Yeast Malt Extract Agar

(YM Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

Glucose ....................................................................................... 10.0g

Peptone.......................................................................................... 5.0g

Yeast extract.................................................................................. 3.0g

Malt extract ................................................................................... 3.0g

pH 6.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. The medium may be rendered selective by adjust

ing the pH to 3.0–4.0 at 45°–55°C or by the addition of antibiotics at

45°–50°C or below. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of fungi, including yeasts, and other aciduric

microorganisms such as Actinoplanes species, Streptomyces species,

Streptoverticillium species, and Nocardia species.