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Yeast Malt Extract Broth with 1.0% Methanol (YM Broth with 1.0% Methanol)

[所属分类:培养基配方] [发布时间:2021-10-20] [发布人:网站管理员2] [阅读次数:] [返回]

Yeast Malt Extract Broth with 1.0% Methanol

(YM Broth with 1.0% Methanol)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose ....................................................................................... 10.0g

Peptone.......................................................................................... 5.0g

Yeast extract.................................................................................. 3.0g

Malt extract ................................................................................... 3.0g

Methanol ..................................................................................10.0mL

pH 6.2 ± 0.2 at 25°C

Preparation of Medium: Add components, except methanol, to

distilled/deionized water and bring volume to 990.0mL. Mix thorough

ly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room tem

perature. Filter sterilize the methanol. Aseptically add the sterile

methanol to the cooled sterile basal medium. Mix thoroughly. Distrib

ute into sterile flasks or tubes.

Use: For the cultivation and maintenance of Pichia angusta and Wick

erhamiella domercqiae.