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Yeast Malt Extract Catalase Agar (YM Catalase Agar)

[所属分类:培养基配方] [发布时间:2021-10-20] [发布人:网站管理员2] [阅读次数:] [返回]

Yeast Malt Extract Catalase Agar

(YM Catalase Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

K2HPO4....................................................................................... 5.74g

Malt extract................................................................................... 5.0g

Yeast extract.................................................................................. 5.0g

NH4H2PO4 .................................................................................. 1.15g

Magnesium sulfate solution.....................................................10.0mL

Catalase solution......................................................................10.0mL

Catalase Solution:

Composition per 10.0mL:

Catalase.................................................................................... 60.0mg

Preparation of Catalase Solution: Add catalase to distilled/de

ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster

ilize.

Magnesium Sulfate Solution:

Composition per 10.0mL:

MgSO4·7H2O......................................................................... 205.0mg

Preparation of Magnesium Sulfate Solution: Add

MgSO4·7H2O to distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except catalase and

magnesium sulfate solutions, to distilled/deionized water and bring

volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 50°C. Aseptically add filter-sterilized catalase and

magnesium sulfate solutions.

Use: For the cultivation and maintenance of Rarobacter faecitabidus.