培养基配方

您现在的位置：网站首页 >> 培养基配方

Yeast Mannitol Agar

[所属分类:培养基配方] [发布时间:2021-10-20] [发布人:网站管理员2] [阅读次数:] [返回]

Yeast Mannitol Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

Mannitol...................................................................................... 10.0g

Na2HPO4·12H2O........................................................................... 1.2g

KH2PO4....................................................................................... 0.55g

MgSO4·7H2O .............................................................................. 0.25g

NaCl............................................................................................ 0.25g

Yeast extract................................................................................ 0.25g

CaSO4·2H2O ............................................................................ 30.0mg

FeSO4·7H2O............................................................................... 3.5mg

H3BO3 .....................................................................................500.0μg

MnSO4·4H2O ..........................................................................400.0μg

ZnSO4·7H2O ...........................................................................160.0μg

CuSO4·5H2O.............................................................................80.0μg

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of Bradyrhizobium japonicum and Rhizobium

leguminosarum.