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Yeast Synthetic Medium with 5′-dTMP

[所属分类:培养基配方] [发布时间:2021-10-21] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose ....................................................................................... 20.0g

Agar ............................................................................................ 15.0g

Yeast nitrogen base without amino acids...................................... 6.7g

Casamino acids without vitamins ................................................. 2.0g

2′-Deoxythymidine-5′-monophosphate .................................... 7.2mg

Preparation of Medium: Add glucose, yeast nitrogen base without

amino acids, casamino acids without vitamins, and 2′-deoxythymi

dine-5′-monophosphate to distilled/deionized water and bring volume

to 200.0mL. Mix thoroughly. Filter sterilize. Add agar to distilled/de

ionized water and bring volume to 800.0mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 50°C. Aseptically combine the two sterile solutions.

Mix thoroughly. Pour into sterile Petri dishes or aseptically distribute

into sterile tubes.

Use: For the cultivation and maintenance of Saccharomyces cerevisiae.