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Yeast Synthetic Minimal Medium

[所属分类:培养基配方] [发布时间:2021-10-21] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose ....................................................................................... 20.0g

Agar ............................................................................................ 15.0g

Yeast nitrogen base without amino acids...................................... 6.7g

Preparation of Medium: Add glucose and yeast nitrogen base

without amino acids to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize. Add agar to distilled/deion

ized water and bring volume to 900.0mL. Mix thoroughly. Gently heat

and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C.

Cool to 50°C. Aseptically combine the two sterile solutions. Mix thor

oughly. Pour into sterile Petri dishes or aseptically distribute into sterile

tubes.

Use: For the cultivation and maintenance of Pichia angusta and Sac

charomyces cerevisiae.