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YEME Thiostrepton Medium

[所属分类:培养基配方] [发布时间:2021-10-21] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sucrose...................................................................................... 340.0g

Peptone.......................................................................................... 5.0g

Malt extract ................................................................................... 3.0g

Yeast extract.................................................................................. 3.0g

Thiostrepton solution ...............................................................10.0mL

MgCl2·6H2O solution.................................................................2.0mL

Thiostrepton Solution:

Composition per 10.0mL:

Thiostrepton .............................................................................10.0mg

Preparation of Thiostrepton Solution: Add thiostrepton to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

MgCl2·6H2O Solution:

Composition per 100.0mL:

MgCl2·6H2O................................................................................ 50.8g

Preparation of MgCl2·6H2O Solution: Add MgCl2·6H2O to dis

tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Add components, except thiostrepton so

lution and MgCl2·6H2O solution, to distilled/deionized water and bring

volume to 990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 50°C. Aseptically add 10.0mL of sterile

thiostrepton solution and 2.0mL of sterile solution. Mix thoroughly.

Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Streptomyces lividans.