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Yersinia Isolation HiVeg Agar

[所属分类:培养基配方] [发布时间:2021-10-21] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Plant peptone............................................................................... 15.0g

Lactose ........................................................................................ 10.0g

Sodium citrate ............................................................................. 10.0g

Plant extract No. 1 ........................................................................ 8.5g

Na2S2O3 ........................................................................................ 8.5g

Yeast extract.................................................................................. 5.0g

Synthetic detergent No. II............................................................. 3.0g

Synthetic detergent No. III............................................................ 2.0g

CaCl2............................................................................................. 1.0g

Ferric citrate.................................................................................. 1.0g

Neutral Red ............................................................................... 0.025g

Brilliant Green ...........................................................................0.3mg

pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Heat to boiling. Do

not autoclave. Pour into sterile Petri dishes.

Use: For the isolation and characterization of Yersinia enterocolitica

from fecal specimens and enumeration of Yersinia enterocolitica from

water and other liquid specimens.