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Yersinia Selective Agar Base

[所属分类:培养基配方] [发布时间:2021-10-22] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Mannitol...................................................................................... 20.0g

Peptone........................................................................................ 17.0g

Agar ............................................................................................ 12.5g

Proteose peptone ........................................................................... 3.0g

Yeast extract.................................................................................. 2.0g

Sodium pyruvate ........................................................................... 2.0g

NaCl .............................................................................................. 1.0g

Sodium desoxycholate .................................................................. 0.5g

MgSO4·7H2O .............................................................................. 0.01g

Neutral Red ................................................................................. 0.03g

Crystal Violet .............................................................................1.0mg

Selective supplement .................................................................6.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems and Oxoid Unipath.

Selective Supplement:

Composition per 6.0mL:

Cefsulodin................................................................................15.0mg

Irgasan........................................................................................4.0mg

Novobiocin.................................................................................2.5mg

Ethanol .......................................................................................2.0mL

Preparation of Selective Supplement: Aseptically add compo

nents to 4.0mL of distilled/deionized water and 2.0mL of ethanol. Mix

thoroughly.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 50°C. Aseptically add selective supple

ment. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.

Use: For the isolation and enumeration of Yersinia enterocolitica from

food and clinical specimens.