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YGC Medium with Cysteine (Yeast Extract Glucose Citrate Medium with Cysteine)

[所属分类:培养基配方] [发布时间:2021-10-22] [发布人:网站管理员2] [阅读次数:] [返回]

YGC Medium with Cysteine

(Yeast Extract Glucose

Citrate Medium with Cysteine)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose ....................................................................................... 10.0g

Peptone........................................................................................ 10.0g

Beef extract ................................................................................. 10.0g

Yeast extract.................................................................................. 5.0g

Ammonium citrate ........................................................................ 5.0g

Sodium acetate.............................................................................. 2.0g

Tween™ 80................................................................................... 1.0g

MgSO4·7H2O ................................................................................ 0.2g

MnSO4·4H2O .............................................................................. 0.05g

L-Cysteine·HCl·H2O solution ..................................................10.0mL

pH 6.5 ± 0.2 at 25°C

L-Cysteine·HCl Solution:

Composition per 10.0mL:

L-Cysteine·HCl·H2O ..................................................................... 0.5g

Preparation of L-Cysteine·HCl Solution: Add L-cyste

ine·HCl·H2O to distilled/deionized water and bring volume to 10.0mL. Mix

thoroughly. Filter sterilize.

Preparation of Medium: Add components, except for the L-cyste

ine·HCl·H2O, to distilled/deionized water and bring volume to

990.0mL. Mix thoroughly. Distribute into tubes or flasks. Autoclave

for 15 min at 15 psi pressure–121°C. Aseptically add L-cysteine hydro

chloride solution.

Use: For the cultivation and maintenance of Leuconostoc mesenteroi

des.