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YGCP Medium (Yeast Extract Glucose Carbonate Peptone Medium)

[所属分类:培养基配方] [发布时间:2021-10-22] [发布人:网站管理员2] [阅读次数:] [返回]

YGCP Medium

(Yeast Extract Glucose Carbonate Peptone Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose ....................................................................................... 20.0g

Agar ............................................................................................ 17.5g

CaCO3 ......................................................................................... 10.0g

Yeast extract.................................................................................. 2.5g

Peptone ......................................................................................... 2.5g

NaCl.............................................................................................. 1.0g

K2HPO4......................................................................................... 1.0g

MgSO4 .......................................................................................... 0.5g

Preparation of Medium: Add components, except calcium carbon

ate, to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly. Gently heat and bring to boiling. Adjust pH to 7.2. Add

calcium carbonate. Mix thoroughly. Distribute into tubes or flasks. Au

toclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri

dishes or leave in tubes.

Use: For the cultivation and maintenance of Xanthomonas campestris

and Xanthomonas oryzae.