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YMA Agar

[所属分类:培养基配方] [发布时间:2021-10-22] [发布人:网站管理员2] [阅读次数:] [返回]

YMA Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Mannitol...................................................................................... 10.0g

CaCO3 ........................................................................................... 4.0g

KH2PO4......................................................................................... 0.5g

Yeast extract.................................................................................. 0.4g

MgSO4·7H2O................................................................................ 0.2g

NaCl.............................................................................................. 0.1g

pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of Rhizobium fredii, Rhizobium galegae, Rhizo

bium huakuii, Rhizobium leguminosarum, Rhizobium loti, Rhizobium

meliloti, Rhizobium phaseoli, and Rhizobium trifolii.