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YPDP Medium with 5′-TMP

[所属分类:培养基配方] [发布时间:2021-10-22] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose ....................................................................................... 20.0g

Peptone ....................................................................................... 20.0g

Agar ............................................................................................ 15.0g

Yeast extract................................................................................ 10.0g

KH2PO4......................................................................................... 1.5g

Thymidine-5′-monophosphate solution...................................10.0mL

Thymidine-5′-Monophosphate Solution:

Composition per 10.0mL:

Thymidine-5′-monophosphate .............................................. 100.0mg

Preparation of Thymidine-5′-Monophosphate Solution: Add

thymidine-5′-monophosphate to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except thymidine-5′-

monophosphate solution, to distilled/deionized water and bring vol

ume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Asepti

cally add 10.0mL of sterile thymidine-5′-monophosphate solution.

Mix thoroughly. Pour into sterile Petri dishes or aseptically distribute

into sterile tubes.

Use: For the cultivation and maintenance of Saccharomyces cerevi

siae.