培养基配方

您现在的位置：网站首页 >> 培养基配方

YPNC Medium

[所属分类:培养基配方] [发布时间:2021-10-25] [发布人:网站管理员2] [阅读次数:] [返回]

YPNC Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 18.0g

NaCl............................................................................................ 2.92g

KH2PO4..................................................................................... 0.596g

Yeast extract.................................................................................. 0.5g

Sodium hydrogen glutamate (pH 6.0) ........................................ 0.37g

K2HPO4..................................................................................... 0.107g

NH4Cl ....................................................................................... 0.107g

MgSO4·7H2O............................................................................ 0.049g

Glucose solution ......................................................................10.0mL

Trace metals solution .................................................................1.0mL

pH 6.0 ± 0.2 at 25°C

Glucose Solution:

Composition per 10.0mL:

Glucose ......................................................................................... 4.0g

Preparation of Glucose Solution: Add glucose to distilled/deion

ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril

ize.

Trace Metals Solution:

Composition per liter:

EDTA.......................................................................................... 50.0g

ZnSO4·7H2O ............................................................................... 22.0g

CaCl2........................................................................................... 5.54g

MnCl2·4H2O ............................................................................... 5.06g

FeSO4·7H2O................................................................................ 4.99g

(NH4)6Mo7O14·H2O .................................................................... 1.10g

CoSO4·5H2O............................................................................... 1.57g

CoCl2·6H2O ................................................................................ 1.61g

Preparation of Trace Metals Solution: Add components, one at

a time, to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly. Filter sterilize.

Preparation of Medium: Add components, except glucose solu

tion and trace metals solution, to distilled/deionized water and bring

volume to 989.0mL. Mix thoroughly. Gently heat and bring to boiling.

Adjust pH to 6.0 with KOH. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 50°C. Aseptically add 10.0mL of sterile glucose solu

tion and 1.0mL of sterile trace metals solution. Mix thoroughly. Pour

into sterile Petri dishes or aseptically distribute into sterile tubes.

Use: For the cultivation and maintenance of a variety of Cryptococcus

species.