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YPSC Medium (Yeast Extract Peptone Sulfate Cysteine Medium)

[所属分类:培养基配方] [发布时间:2021-10-25] [发布人:网站管理员2] [阅读次数:] [返回]

YPSC Medium

(Yeast Extract Peptone Sulfate Cysteine Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract.................................................................................. 1.0g

Peptone ......................................................................................... 1.0g

Sodium acetate·3H2O.................................................................... 0.5g

MgSO4·7H2O.............................................................................. 0.25g

CaCl2·2H2O ................................................................................ 0.25g

L-Cysteine·HCl·H2O ................................................................... 0.05g

pH 7.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes

or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Adjust pH to

7.5 with sterile 10M NaOH.

Use: For the cultivation and enumeration of Bdellovibrio species.