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Zavarzinella formosa Medium (DSMZ Medium 1196)

[所属分类:培养基配方] [发布时间:2021-10-25] [发布人:网站管理员2] [阅读次数:] [返回]

Zavarzinella formosa Medium

(DSMZ Medium 1196)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

N-acetylglucosamine ................................................................... 1.0g

Glucose ........................................................................................ 0.5g

KH2PO4 ........................................................................................ 0.1g

Peptone ........................................................................................ 0.1g

Yeast extract ................................................................................. 0.1g

MgSO4·7H2O................................................................................ 0.1g

Casamino acids ............................................................................ 0.1g

CaCl2·2H2O ................................................................................ 0.05g

NaCl ........................................................................................... 0.01g

“Metals 44”................................................................................1.0mL

pH 5.9 ± 0.2 at 25°C

“Metals 44”:

Composition per 100.0mL:

ZnSO4·7H2O ............................................................................. 1.095g

FeSO4·7H2O.................................................................................. 0.5g

Sodium EDTA............................................................................. 0.25g

MnSO4·H2O.............................................................................. 0.154g

CuSO4·5H2O ............................................................................39.2mg

Co(NO3)2·6H2O .......................................................................24.8mg

Na2B4O7·10H2O.......................................................................17.7mg

Preparation of “Metals 44”: Add sodium EDTA to distilled/de

ionized water and bring volume to 90.0mL. Mix thoroughly. Add a few

drops of concentrated H2SO4 to retard precipitation of heavy metal

ions. Add remaining components. Mix thoroughly. Bring volume to

100.0mL with distilled/deionized water.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 5.8–6.0.

Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–

121°C.

Use: For the cultivation of Zavarzinella formosa.