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A-1 Medium (BAM M1)

[所属分类:培养基配方] [发布时间:2021-10-27] [发布人:网站管理员2] [阅读次数:] [返回]

A-1 Medium

(BAM M1)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................20.0g

Lactose................................................................5.0g

NaCl....................................................................5.0g

Salicin .................................................................0.5g

Triton™ X-100 ............................................... 1.0mL

pH 6.9 ± 0.1 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Adjust pH to 6.9. Distribute into test

tubes containing an inverted Durham tube. Medium

may be cloudy prior to autoclaving. Autoclave for 10

min at 15 psi pressure–121°C.

Use: For the detection of fecal coliforms in foods

and waters by a most-probable-number (MPN) meth

od. Multiple dilutions of samples (3, 5, or 10 repli

cates per dilution) are added to tubes containing A-1

medium. After incubation, test tubes with gas accu

mulation in the Durham tubes are scored positive and

those with no gas as negative. A MPN table is con

sulted to determine the most probable number of fe

cal coliforms.