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ABY Agar (Acid Bismuth Yeast Agar)

[所属分类:培养基配方] [发布时间:2021-10-27] [发布人:网站管理员2] [阅读次数:] [返回]

ABY Agar

(Acid Bismuth Yeast Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................20.0g

Glucose .............................................................20.0g

Bi2(SO3)2.............................................................8.0g

(NH4)2SO4...........................................................3.0g

KH2PO4...............................................................3.0g

MgSO4·7H2O ....................................................0.25g

CaCl2·2H2O.......................................................0.25g

Biotin ............................................................. 10.0μg

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Cool tubes in a slanted po

sition.

Use: For the selective isolation and differentiation of

Candida albicans, which can cause food allergies,

from other Candida species. Candida albicans and

Candida tropicalis colonies appear as smooth,

brownish-black round colonies. Other Candida spe

cies are differentially pigmented or produce diffus

ible pigments. Usually used in conjunction with BiG

GY agar to differentiate further Candida; on BiGGY

agar, Candida albicans appears as brown to black

colonies with no pigment diffusion and no sheen,

whereas Candida tropicalis appears as dark brown

colonies with black centers, black pigment diffusion,

and a sheen.