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Acetobacter Medium

[所属分类:培养基配方] [发布时间:2021-10-27] [发布人:网站管理员2] [阅读次数:] [返回]

Acetobacter Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Autolyzed yeast.................................................10.0g

CaCO3 ...............................................................10.0g

Glucose ...............................................................3.0g

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes to produce a 1cm butt and 30cm

slant. Autoclave for 15 min at 15 psi pressure–121°C.

Agitate tubes to mix CaCO3. Cool tubes rapidly in a

slanted position to keep the CaCO3 in suspension.

Use: For the cultivation and maintenance of Aceto

bacter species and Gluconobacter species.