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Acid Egg Medium

[所属分类:培养基配方] [发布时间:2021-10-27] [发布人:网站管理员2] [阅读次数:] [返回]

Acid Egg Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1640.0mL:

Potato starch......................................................30.0g

KH2PO4.............................................................12.3g

Malachite Green..................................................0.4g

MgSO4·7H2O ......................................................0.3g

Penicillin G .............................................. 100,000IU

Fresh egg mixture ..................................... 1000.0mL

Glycerol ........................................................ 12.0mL

Source: This medium is available as a prepared me

dium from Oxoid.

Preparation of Medium: Add components to

1.0L of fresh egg mixture. Mix thoroughly. Gently

heat and bring to boiling. Bring volume to 1640.0mL

with distilled/deionized water. Distribute into tubes

or flasks. Autoclave for 15 min at 15 psi pressure–

121°C with tubes in an upright position.

Use: For the cultivation and maintenance of Myco

bacterium tuberculosis.