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Acid Tomato Broth

[所属分类:培养基配方] [发布时间:2021-10-27] [发布人:网站管理员2] [阅读次数:] [返回]

Acid Tomato Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................10.0g

Peptone .............................................................10.0g

Yeast extract........................................................5.0g

MgSO4·7H2O......................................................0.2g

MnSO4·4H2O....................................................0.05g

Tomato juice ............................................... 250.0mL

L-Cysteine solution ......................................... 0.5mL

L-Cysteine Solution:

Composition per 10.0mL:

L-Cysteine ...........................................................0.1g

Preparation of L-Cysteine Solution: Add 0.1g

of L-cysteine to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept L-cysteine solution, to distilled/deionized water

and bring volume to 999.5mL. Mix thoroughly. Ad

just pH to 4.8. Autoclave for 15 min at 15 psi pres

sure–121°C. Aseptically add 0.5mL of sterile L

cysteine solution. Mix thoroughly. Aseptically dis

tribute into sterile tubes or flasks.

Use: For the cultivation of a variety of fungi.