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Acidophilic Bacillus stearothermophilus Agar

[所属分类:培养基配方] [发布时间:2021-10-27] [发布人:网站管理员2] [阅读次数:] [返回]

Acidophilic Bacillus

stearothermophilus Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Part A .......................................................... 400.0mL

Part B .......................................................... 600.0mL

pH 5.0 ± 0.2 at 25°C

Part A:

Composition per 400.0mL:

Soluble starch....................................................10.0g

Pancreatic digest of casein..................................5.0g

Yeast extract........................................................5.0g

KH2PO4...............................................................1.0g

CaCl2·2H2O.........................................................0.5g

MnCl2·4H2O .......................................................0.5g

Preparation of Part A: Add components to dis

tilled/deionized water and bring volume to 400.0mL.

Mix thoroughly. Gently heat and bring to boiling.

Adjust pH to 4.7. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 50°C.

Part B:

Composition per 600.0mL:

Agar ..................................................................20.0g

Preparation of Part B: Add agar to distilled/

deionized water and bring volume to 600.0mL. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

50°C.

Preparation of Medium: Aseptically combine

solution A and solution B. Mix thoroughly. Adjust

pH to 5.0. Pour into sterile Petri dishes.

Use: For the cultivation and maintenance of Bacillus

stearothermophilus and other acidophilic Bacillus

species.