培养基配方

您现在的位置：网站首页 >> 培养基配方

Alkaline Bacillus Medium

[所属分类:培养基配方] [发布时间:2021-10-28] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Peptone..............................................................10.0g

Glucose .............................................................10.0g

Yeast extract........................................................5.0g

K2HPO4...............................................................1.0g

Na2CO3 solution ......................................... 100.0mL

pH 8.5–11.0 at 25°C

Na2CO3 Solution:

Composition per 100.0mL:

Na2CO3 .............................................................10.0g

Preparation of Na2CO3 Solution: Add Na2CO3

to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept Na2CO3 solution, to distilled/deionized water

and bring volume to 900.0mL. Gently heat while stir

ring and bring to boiling. Autoclave for 15 min at 10

psi pressure–115°C. Cool to 45°–50°C. Aseptically

add sterile Na2CO3 solution. Mix thoroughly. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of alkalo

philic microorganisms such as Bacillus alcalophilus,

Bacillus circulans, and other Bacillus species.