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Antibiotic Medium 1 with Tetracycline, Streptomycin, and Chloramphenicol

[所属分类:培养基配方] [发布时间:2021-11-1] [发布人:网站管理员2] [阅读次数:] [返回]

Antibiotic Medium 1 with Tetracycline,

Streptomycin, and Chloramphenicol

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Pancreatic digest of gelatin.................................6.0g

Pancreatic digest of casein..................................4.0g

Yeast extract........................................................3.0g

Beef extract.........................................................1.5g

Glucose ...............................................................1.0g

Antibiotic solution ........................................ 10.0mL

pH 6.6 ± 0.1 at 25°C

Antibiotic Solution:

Composition per 10.0mL:

Tetracycline.......................................................0.02g

Streptomycin.....................................................0.02g

Chloramphenicol...............................................0.02g

Preparation of Antibiotic Solution: Add com

ponents to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept tetracycline solution, to distilled/deionized wa

ter and bring volume to 1.0L. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C. Aseptically

add sterile antibiotic solution. Mix thoroughly. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the selective cultivation and maintenance

of Salmonella choleraesuis.