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APT Broth

[所属分类:培养基配方] [发布时间:2021-11-2] [发布人:网站管理员2] [阅读次数:] [返回]

APT Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................12.5g

Glucose .............................................................10.0g

Yeast extract........................................................7.5g

NaCl....................................................................5.0g

K2HPO4...............................................................5.0g

Sodium citrate.....................................................5.0g

MgSO4·7H2O......................................................0.8g

MnCl2·4H2O .....................................................0.14g

FeSO4·7H2O......................................................0.04g

Polysorbate 80 ....................................................0.2g

Na2CO3 .............................................................1.25g

Thiamine·HCl ..................................................1.0mg

pH 7.7 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi—118°–121°C.

Use: For the cultivation of lactic acid bacteria. For

the cultivation of heterofermentative lactobacilli

from meat and other foods. The American Public

Health Association recommends adding 100.0μg/L

of thiamine to this medium.