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ASLA Agar

[所属分类:培养基配方] [发布时间:2021-11-2] [发布人:网站管理员2] [阅读次数:] [返回]

ASLA Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sodium lactate...................................................20.0g

Davis agar .........................................................10.0g

(NH4)2SO4...........................................................3.0g

Na2HPO4 .............................................................1.2g

L-Cysteine·HCl....................................................0.5g

MgSO4·7H2O ......................................................0.2g

MnSO4·4H2O ....................................................0.05g

FeSO4·7H2O......................................................0.04g

Vitamin solution............................................ 10.0mL

pH 6.5 ± 0.2 at 25°C

Vitamin Solution:

Composition per liter:

Biotin ..................................................................0.1g

Calcium pantothenate .........................................0.1g

p-Aminobenzoic acid..........................................0.1g

Thiamine .............................................................0.1g

Preparation of Vitamin Solution: Add compo

nents to distilled/deionized water and bring volume

to 1.0L. Mix thoroughly. Filter sterilize. Store solu

tion at −20°C.

Preparation of Medium: Add components, except

vitamin solution, to distilled/deionized water and bring

volume to 990.0mL. Mix thoroughly. Gently heat and

bring to boiling. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL

of sterile vitamin solution. Mix thoroughly. Pour into

sterile Petri dishes or distribute into sterile tubes.

Use: For the selective isolation and cultivation of

most Propionibacterium species from foods.