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Bacillus Agar

[所属分类:培养基配方] [发布时间:2021-11-2] [发布人:网站管理员2] [阅读次数:] [返回]

Bacillus Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................20.0g

(NH4)2SO4 ..........................................................1.3g

Glucose ...............................................................1.0g

Yeast extract........................................................1.0g

KH2PO4.............................................................0.37g

MgSO4·7H2O....................................................0.25g

CaCl2·2H2O ......................................................0.07g

FeCl3 .................................................................0.02g

pH 4.0 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to

500.0mL. Mix thoroughly. Gently heat and bring to

boiling. Adjust pH to 3.5. Prepare a separate agar so

lution by adding 20.0g/500.0mL of distilled/deion

ized water. Autoclave solutions separately for 15 min

at 15 psi pressure–121°C. Cool to 50°–55°C. Asepti

cally combine both solutions. This procedure avoids

acid hydrolysis of the agar. Pour into sterile Petri

dishes or leave in tubes.

Use: For the cultivation of acidophilic Bacillus spe

cies such as Bacillus acidocaldarius.