培养基配方

您现在的位置：网站首页 >> 培养基配方

Bacillus cereus Medium (BCM)

[所属分类:培养基配方] [发布时间:2021-11-2] [发布人:网站管理员2] [阅读次数:] [返回]

Bacillus cereus Medium

(BCM)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 110.0mL:

Agar ....................................................................2.0g

D-Mannitol ..........................................................1.0g

(NH4)2PO4...........................................................0.1g

KCl....................................................................0.02g

MgSO4·7H2O ....................................................0.02g

Yeast extract......................................................0.02g

Bromcresol Purple ...........................................4.0mg

Egg yolk emulsion, 20%............................... 10.0mL

pH 7.0 ± 0.2 at 25°C

Egg Yolk Emulsion, 20%:

Composition per 100.0mL:

Chicken egg yolks..................................................11

Whole chicken egg...................................................1

NaCl (0.9% solution) .................................... 80.0mL

Preparation of Egg Yolk Emulsion, 20%:

Soak eggs with 1:100 dilution of saturated mercuric

chloride solution for 1 min. Crack eggs and separate

yolks from whites. Mix egg yolks with 1 chicken egg.

Measure 20.0mL of egg yolk emulsion and add to

80.0mL of 0.9% NaCl solution. Mix thoroughly. Fil

ter sterilize. Warm to 45°–50°C.

Preparation of Medium: Add components—ex

cept egg yolk emulsion, 20%—to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add 10.0mL of sterile egg yolk emulsion,

20%. Mix thoroughly. Pour into sterile Petri dishes or

distribute into sterile tubes.

Use: For the cultivation of Bacillus cereus.