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Bacillus coagulans Medium

[所属分类:培养基配方] [发布时间:2021-11-2] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................20.0g

Glucose ...............................................................5.0g

Proteose peptone.................................................5.0g

Yeast extract........................................................5.0g

K2HPO4...............................................................4.0g

MnSO4·4H2O solution .................................. 10.0mL

CaCl2 solution............................................... 10.0mL

pH 5.0 ± 0.2 at 25°C

MnSO4·4H2O Solution:

Composition per 10.0mL:

MnSO4·4H2O .................................................0.05mg

Preparation of MnSO4·4H2O Solution: Add

MnSO4·4H2O to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

CaCl2 Solution:

Composition per 10.0mL:

CaCl2............................................................0.045mg

Preparation of CaCl2 Solution: Add CaCl2 to

distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept MnSO4·4H2O solution and CaCl2 solution, to

distilled/deionized water and bring volume to

980.0mL. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Avoid overheating. Cool to 45°–50°C. Asep

tically add sterile MnSO4·4H2O solution and CaCl2

solution. Mix thoroughly. Pour into sterile Petri dish

es or distribute into sterile tubes.

Use: For the cultivation of Bacillus coagulans.