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Bacillus stearothermophilus Sporulation Broth

[所属分类:培养基配方] [发布时间:2021-11-3] [发布人:网站管理员2] [阅读次数:] [返回]

Bacillus stearothermophilus

Sporulation Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................20.0g

Pancreatic digest of gelatin.................................5.0g

Yeast extract........................................................4.0g

Beef extract.........................................................3.0g

MnCl2·4H2O ...................................................10.0μg

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Pour into sterile Petri dish

es or leave in tubes.

Use: For the cultivation and sporulation of Bacillus

stearothermophilus.