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BAGG Broth (Buffered Azide Glucose Glycerol Broth)

[所属分类:培养基配方] [发布时间:2021-11-3] [发布人:网站管理员2] [阅读次数:] [返回]

BAGG Broth

(Buffered Azide Glucose Glycerol Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................10.0g

Peptic digest of animal tissue ...........................10.0g

Glucose ...............................................................5.0g

NaCl....................................................................5.0g

K2HPO4...............................................................4.0g

KH2PO4...............................................................1.5g

NaN3 ...................................................................0.5g

Bromcresol Purple ..........................................0.015g

Glycerol .......................................................... 5.0mL

pH 6.9 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Caution: Sodium azide is toxic. Azides also react

with metals and disposal must be highly diluted.

Preparation of Medium: Add 5.0mL of glycerol

to 900.0mL of distilled/deionized water. Add remain

ing components and bring volume to 1.0L. Mix thor

oughly. Gently heat and bring to boiling. Distribute

into tubes in 10.0mL volumes. Autoclave for 15 min

at 10 psi pressure–116°C.

Use: For the cultivation of fecal streptococci. It is

recommended for qualitative presumptive and con

firmatory tests for fecal streptococci.