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Biotin Assay Medium

[所属分类:培养基配方] [发布时间:2021-11-3] [发布人:网站管理员2] [阅读次数:] [返回]

Biotin Assay Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................40.0g

Sodium acetate..................................................20.0g

Vitamin assay casamino acids ..........................12.0g

K2HPO4...............................................................1.0g

KH2PO4...............................................................1.0g

MgSO4·7H2O......................................................0.4g

DL-Tryptophan.....................................................0.2g

L-Cystine .............................................................0.2g

Adenine sulfate .................................................0.02g

FeSO4................................................................0.02g

Guanine·HCl .....................................................0.02g

MgSO4·7H2O....................................................0.02g

NaCl..................................................................0.02g

Uracil ................................................................0.02g

Calcium pantothenate ......................................2.0mg

Niacin...............................................................2.0mg

Pyridoxine·HCl ................................................2.0mg

Riboflavin ........................................................2.0mg

Thiamine·HCl ..................................................2.0mg

p-Aminobenzoic acid.......................................0.2mg

pH 6.7 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Continue boiling for 2–3 min. Distribute into tubes in

5.0mL volumes. Add standard solution or test solu

tions to each tube. Adjust the volume of each tube to

10.0mL with distilled/deionized water. Autoclave for

15 min at 15 psi pressure–121°C.

Use: For use in the microbiological assay of biotin

using Lactobacillus plantarum as the test microor

ganism.