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Bovine Albumin Tween 80 Medium, Ellinghausen and McCullough, Modi fied (Albumin Fatty Acid Broth, Leptospira Medium)

[所属分类:培养基配方] [发布时间:2021-11-5] [发布人:网站管理员2] [阅读次数:] [返回]

Bovine Albumin Tween 80 Medium,

Ellinghausen and McCullough, Modi

fied

(Albumin Fatty Acid Broth,

Leptospira Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Basal medium ............................................. 900.0mL

Albumin fatty acid supplement................... 100.0mL

Basal Medium:

Composition per liter:

Na2HPO4, anhydrous ..........................................1.0g

NaCl....................................................................1.0g

KH2PO4, anhydrous ............................................0.3g

NH4Cl (25% solution)..................................... 1.0mL

Glycerol (10% solution).................................. 1.0mL

Sodium pyruvate (10% solution) .................... 1.0mL

Thiamine·HCl (0.5% solution)........................ 1.0mL

pH 7.4 ± 0.2 at 25°C

Preparation of Basal Medium: Add compo

nents to distilled/deionized water and bring volume

to 1.0L. Mix thoroughly. Adjust pH to 7.4. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 25°C.

Albumin Fatty Acid Supplement:

Composition per 200.0mL:

Bovine albumin fraction V ...............................20.0g

Polysorbate (Tween ) 80 (10% solution) ...... 25.0mL

FeSO4·7H2O (0.5% solution)........................ 20.0mL

CaCl2·2H2O (1.5% solution)........................... 2.0mL

MgCl2·2H2O (1.5% solution) ......................... 2.0mL

Vitamin B12 (0.2% solution) ........................... 2.0mL

ZnSO4·7H2O (0.4% solution) ......................... 2.0mL

CuSO4·5H2O (0.3% solution) ......................... 0.2mL

Preparation of Albumin Fatty Acid Supple

ment: Add bovine albumin to 100.0mL of distilled/

deionized water. Mix thoroughly. Add remaining

components while stirring. Adjust pH to 7.4. Bring

volume to 200.0mL with distilled/deionized water.

Filter sterilize. Store at −20°C.

Preparation of Medium: Aseptically combine

100.0mL of sterile albumin fatty acid supplement

and 900.0mL of sterile basal medium. Mix thorough

ly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Leptospira species.

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Bovine Albumin Tween 80 Medium, Ellinghausen and McCullough, Modi fied (Albumin Fatty Acid Broth, Leptospira Medium)