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Brain Heart Infusion Agar 0.7% (BHI Agar 0.7%) (BAM M23)

[所属分类:培养基配方] [发布时间:2021-11-5] [发布人:网站管理员2] [阅读次数:] [返回]

Brain Heart Infusion Agar 0.7%

(BHI Agar 0.7%)

(BAM M23)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of gelatin...............................14.5g

Agar ....................................................................7.0g

Brain heart, solids from infusion ........................6.0g

Peptic digest of animal tissue..............................6.0g

NaCl....................................................................5.0g

Glucose ...............................................................3.0g

Na2HPO4 .............................................................2.5g

pH 5.3 ± 0.2 at 25°C

Source: This medium without agar is available as a

premixed powder from BD Diagnostic Systems.

Preparation of Medium: Add components, ex

cept agar, to distilled/deionized water and bring vol

ume to 1.0L. Mix thoroughly. Adjust pH to 5.3 with

1N HCl. Mix thoroughly. Add agar. Gently heat and

bring to boiling. Distribute into tubes. Autoclave for

10 min at 15 psi pressure–121°C.

Use: For the detection of staphylococcal enterotoxin.