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Brigg’s Liver Tomato Broth

[所属分类:培养基配方] [发布时间:2021-11-5] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................20.0g

Neopeptone .......................................................15.0g

Yeast extract........................................................6.0g

NaCl....................................................................5.0g

Tween 80.............................................................1.0g

Soluble starch......................................................0.5g

L-Cysteine·HCl ...................................................0.2g

Tomato juice ............................................... 400.0mL

Liver extract.................................................. 75.0mL

pH 5.0 ± 0.2 at 25°C

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. (Note:

3.0g of proteolysed liver may be used instead of the liv

er extract.) Mix thoroughly. Distribute into tubes or

flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Lactobacillus acetotolerans,

a bacterium found in fermented vinegar broth.