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Brilliant Green Agar, Modified (Edel-Kampelmacher Medium)

[所属分类:培养基配方] [发布时间:2021-11-5] [发布人:网站管理员2] [阅读次数:] [返回]

Brilliant Green Agar, Modified

(Edel-Kampelmacher Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................12.0g

Peptone..............................................................10.0g

Lactose..............................................................10.0g

Sucrose..............................................................10.0g

Lab-Lemco..........................................................5.0g

Yeast extract........................................................3.0g

Na2HPO4 .............................................................1.0g

NaH2PO4 .............................................................0.6g

Phenol Red........................................................0.09g

Brilliant Green ..............................................0.0047g

Sulphamandelate supplement ....................... 10.0mL

pH 6.9 ± 0.2 at 25°C

Sulphamandelate Supplement:

Composition per 10.0mL:

Sodium sulphacetamide ......................................1.0g

Sodium mandelate.............................................0.25g

Preparation of Sulphamandelate Supple

ment: Add components to distilled/deionized water

and bring volume to 10.0mL. Mix thoroughly. Filter

sterilize.

Source: This medium is available as a premixed

powder from Oxoid.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to

990.0mL. Mix thoroughly. Gently heat and bring to a

boil to dissolve the components completely. Do not

autoclave. Cool to 50°C. Aseptically add 10.0mL

sulphamandelate supplement. Mix thoroughly. Pour

into sterile Petri dishes or leave in tubes.

Use: For the selective isolation and differentiation of

Salmonella spp. other than S. typhi.