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Brilliant Green Lactose Bile Broth (BAM M25)

[所属分类:培养基配方] [发布时间:2021-11-5] [发布人:网站管理员2] [阅读次数:] [返回]

Brilliant Green Lactose Bile Broth

(BAM M25)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Oxgall, dehydrated............................................20.0g

Lactose..............................................................10.0g

Pancreatic digest of gelatin...............................10.0g

Brilliant Green ..............................................0.0133g

pH 7.2 ± 0.1 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems and Oxoid.

Preparation of Medium: Add lactose and pan

creatic digest of gelatin to distilled/deionized water

and bring volume to 500.0mL. Mix thoroughly. Add

20.0g oxgall dissolved in 200.0mL distilled/deion

ized water. The pH of this solution should be 7.0-7.5.

Mix thoroughly. Bring volume to 975.0mL with dis

tilled/deionized water. Adjust pH to 7.4. Add 13.3mL

of 0.1% aqueous Brilliant Green in distilled/deion

ized water. Adjust volume to 1.0L with distilled/

deionized water. Distribute into tubes containing in

verted Durham tubes, in 10.0mL amounts for testing

1.0mL or less of sample. Make sure that the fluid lev

el covers the inverted vials. Autoclave for 15 min at

15 psi pressure–121°C. After sterilization, cool the

broth rapidly. Medium is sensitive to light.The final

pH should be 7.2 ± 0.1 at 25°C.

Use: For the detection of coliform microorganisms

in foods, dairy products, water, and wastewater as

well as in other materials of sanitary importance.

Turbidity in the broth and gas in the Durham tube are

positive indications of Escherichia coli.