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Brilliant Green Bile Broth with MUG

[所属分类:培养基配方] [发布时间:2021-11-9] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Oxgall, dehydrated............................................20.0g

Lactose..............................................................10.0g

Pancreatic digest of gelatin...............................10.0g

MUG (4-Methyl umbelliferyl-

β-D-glucuronide).........................................0.05g

Brilliant Green ................................................0.013g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into tubes containing in

verted Durham tubes, in 10.0mL amounts for testing

1.0mL or less of sample. Autoclave for 12 min (not

longer than 15 min) at 15 psi pressure–121°C. After

sterilization, cool the broth rapidly.

Use: For the detection of coliform microorganisms

in foods, dairy products, water, and wastewater, as

well as in other materials of sanitary importance. The

presence of Eschrichia coli and other coliforms is de

termined by the presence of fluorescence in the tube.