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Bromcresol Purple Broth

[所属分类:培养基配方] [发布时间:2021-11-9] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone .............................................................10.0g

NaCl....................................................................5.0g

Beef extract.........................................................3.0g

Bromcresol Purple ............................................0.04g

Carbohydrate solution................................... 10.0mL

pH 7.0 ± 0.2 at 25°C

Carbohydrate Solution:

Composition per 10.0mL:

Carbohydrate.......................................................5.0g

Preparation of Carbohydrate Solution: Add

carbohydrate to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into test tubes that contain an inverted

Durham tube. Autoclave for 10 min at 15 psi pres

sure–121°C.

Use: For the differentiation of a variety of microor

ganisms based on their fermentation of specific car

bohydrates. Bacteria that ferment the specific

carbohydrate turn the medium yellow. When bacteria

produce gas, the gas is trapped in the Durham tube.