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Brucella Agar with Vitamin K1

[所属分类:培养基配方] [发布时间:2021-11-9] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................17.5g

Pancreatic digest of casein................................10.0g

Peptic digest of animal tissue............................10.0g

NaCl....................................................................5.0g

Yeast extract........................................................2.0g

Glucose ...............................................................1.0g

NaHSO3 ..............................................................0.1g

Sheep blood, defibrinated ............................. 50.0mL

Vitamin K1 solution ........................................ 1.0mL

pH 7.0 ± 0.2 at 25°C

Vitamin K1 Solution:

Composition per 20.0mL:

Vitamin K1 ..........................................................0.2g

Ethanol, absolute........................................... 20.0mL

Preparation of Vitamin K1 Solution: Add vita

min K1 to 20.0mL of ethanol. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components, ex

cept sheep blood and vitamin K1 solution, to dis

tilled/deionized water and bring volume to 949.0mL.

Mix thoroughly. Gently heat and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 45°–50°C. Aseptically add 50.0mL of sterile sheep

blood and 1.0mL of sterile vitamin K1 solution. Mix

thoroughly. Pour into sterile Petri dishes or distribute

into sterile tubes.

Use: For the cultivation of Brucella species.