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Buffered Enrichment Broth (BAM M52)

[所属分类:培养基配方] [发布时间:2021-11-10] [发布人:网站管理员2] [阅读次数:] [返回]

Buffered Enrichment Broth

(BAM M52)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Na2HPO4.............................................................9.6g

KH2PO4.............................................................1.35g

Pyruvic acid solution .................................... 11.1mL

Nalidixic acid solution.................................... 8.0mL

Cyclohiximide solution................................... 5.0mL

Acriflavin solution.......................................... 2.0mL

pH 7.3 ± 0.1 at 25°C

Nalidixic Acid Solution:

Composition per 10.0mL:

Nalidixic acid, sodium salt ...............................0.05g

Preparation of Nalidixic Acid Solution: Add

nalidixic acid to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Acriflavin Solution:

Composition per 10.0mL:

Acriflavin·HCl ..................................................0.05g

Preparation of Acriflavin Solution: Add acri

flavin·HCl to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Cycloheximide Solution:

Composition per 10.0mL:

Cycloheximide....................................................0.1g

Ethanol, 40%................................................. 10.0mL

Preparation of Cycloheximide Solution: Add

cycloheximide to 40% ethanol and bring volume to

10.0mL. Mix thoroughly. Filter sterilize.

Pyruvate Solution:

Composition per 20.0mL:

Na-pyruvate ........................................................2.0g

Preparation of Pyruvate Solution: Add Na

pyruvate to distilled/deionized water and bring vol

ume to 20.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept pyruvate solution, nalidixic acid solution, acri

flavin solution, and cycloheximide solution, to

distilled/deionized water and bring volume to

973.9.0L. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 25°C. Aseptically add 11.1mL sterile

pyruvate solution. Mix thoroughly. Aseptically add

8.0mL sterile nalidixic acid solution, 5.0mL sterile

cycloheximide solution, and 2.0mL sterile acriflavin

solution. Mix thoroughly. Aseptically distribute into

sterile tubes or flasks.

Use: For the cultivation of of Listeria spp.

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Buffered Enrichment Broth (BAM M52)