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Campylobacter Enrichment Broth (FDA Medium M29)

[所属分类:培养基配方] [发布时间:2021-11-10] [发布人:网站管理员2] [阅读次数:] [返回]

Campylobacter Enrichment Broth

(FDA Medium M29)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1020.0mL:

Basal medium ............................................. 950.0mL

Horse blood, lysed ........................................ 50.0mL

FBP solution ................................................... 4.0mL

Cefoperazone solution .................................... 4.0mL

Trimethoprim lactate solution......................... 4.0mL

Vancomycin solution....................................... 4.0mL

Cycloheximide solution .................................. 4.0mL

pH 7.5 ± 0.2 at 25°C

Basal Medium:

Composition per 950.0mL:

Beef extract.......................................................10.0g

Peptone..............................................................10.0g

Yeast extract........................................................6.0g

NaCl....................................................................5.0g

Preparation of Basal Medium: Add compo

nents to distilled/deionized water and bring volume

to 950.0mL. Mix thoroughly. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 45°–50°C.

FBP Solution:

Composition per 100.0mL:

FeSO4·7H2O......................................................6.25g

Na2S2O5 ............................................................6.25g

Sodium pyruvate...............................................6.25g

Preparation of FBP: Add components to dis

tilled/deionized water and bring volume to 100.0mL.

Mix thoroughly. Filter sterilize.

Cefoperazone Solution:

Composition per 10.0mL:

Cefoperazone ..................................................0.037g

Preparation of Cefoperazone Solution: Add

cefoperazone to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Trimethoprim Lactate Solution:

Composition per 10.0mL:

Trimethoprim lactate.......................................0.031g

Preparation of Trimethoprim Lactate Solu

tion: Add trimethoprim lactate to distilled/deionized

water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Vancomycin Solution:

Composition per 10.0mL:

Vancomycin ....................................................0.025g

Preparation of Vancomycin Solution: Add

vancomycin to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Cycloheximide Solution:

Composition per 10.0mL:

Cycloheximide................................................0.025g

Preparation of Cycloheximide Solution: Add

cycloheximide to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: To 950.0mL of cooled,

sterile basal medium, aseptically add 50.0mL of ly

sed (fresh, frozen, and thawed) horse blood, 4.0mL

of sterile FBP solution, 4.0mL of sterile cefopera

zone solution, 4.0mL of sterile trimethoprim lactate

solution, 4.0mL of sterile vancomycin solution, and

4.0mL of sterile cycloheximide solution. Mix thor

oughly. Aseptically distribute into sterile screw

capped tubes or bottles. Close caps tightly to reduce

O2 absorption. Use within 2 weeks.

Use: For the selective isolation and cultivation of

Campylobacter species.

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Campylobacter Enrichment Broth (FDA Medium M29)