编号：
批号：

培养基配方

您现在的位置：网站首页 >> 培养基配方

Campylobacter Enrichment Broth

[所属分类:培养基配方] [发布时间:2021-11-10] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Beef extract.......................................................10.0g

Peptone..............................................................10.0g

Yeast extract........................................................6.0g

NaCl....................................................................5.0g

Horse blood, laked ........................................ 50.0mL

FBP solution ................................................... 4.0mL

Antibiotic solution .......................................... 4.0mL

pH 7.5 ± 0.2 at 25°C

Horse Blood, Laked:

Composition per 50.0mL:

Horse blood, fresh......................................... 50.0mL

Preparation of Horse Blood, Laked: Add

blood to a sterile polypropylene bottle. Freeze over

night at −20°C. Thaw at 8°C. Refreeze at −20°C.

Thaw again at 8°C.

FBP Solution:

Composition per 100.0mL:

FeSO4................................................................6.25g

NaHSO3 ............................................................6.25g

Sodium pyruvate...............................................6.25g

Preparation of FBP Solution: Add components

to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Antibiotic Solution:

Composition per 10.0mL:

Cycloheximide....................................................0.1g

Sodium cefoperazone........................................0.03g

Trimethoprim lactate.....................................0.0125g

Vancomycin ......................................................0.01g

Preparation of Antibiotic Solution: Add com

ponents to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components—ex

cept horse blood, FBP solution, and antibiotic solu

tion—to distilled/deionized water and bring volume

to 942.0mL. Mix thoroughly. Gently heat and bring

to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically add 50.0mL

of sterile horse blood, 4.0mL of FBP solution, and

4.0mL of antibiotic solution. Mix thoroughly. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation and cultivation of Campylo

bacter species from foods.

在线客服

Campylobacter Enrichment Broth