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Cary and Blair Transport Medium (BAM M31)

[所属分类:培养基配方] [发布时间:2021-11-10] [发布人:网站管理员2] [阅读次数:] [返回]

Cary and Blair Transport Medium

(BAM M31)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ....................................................................5.0g

NaCl....................................................................5.0g

Sodium thioglycolate ..........................................1.5g

Na2HPO4 .............................................................1.1g

CaCl2 solution................................................. 9.0mL

pH 8.4 ± 0.5 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems and Oxoid.

CaCl2 Solution:

Composition per 10.0mL:

CaCl2...................................................................0.1g

Preparation of CaCl2 Solution: Add CaCl2 to

distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept CaCl2 solution, to distilled/deionized water and

bring volume to 991.0mL. Mix thoroughly and heat

gently until boiling. Cool to 50°C. Add 9.0mL of a

1% CaCl2 solution. Adjust the pH to 8.4. Distribute

into screw-capped tubes in 7.0mL volumes. Sterilize

under flowing steam for 15 min. After sterilization,

tighten the screwcaps.

Use: For the maintenance—as a holding medium or

transport medium—of specimens during collection

or shipment.