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Cherry Agar, CBS Formula

[所属分类:培养基配方] [发布时间:2021-11-11] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pulp of sour stone cherries..............................200.0g

Agar ..................................................................20.0g

pH 3.8 ± 0.2 at 25°C

Preparation of Medium: Add 1.0L of distilled/

deionized water to pulp of sour stone cherries. Gently

heat and bring to boiling. Simmer gently for 2 hr.

Strain through cloth. Autoclave filtrate for 30 min at

6 psi pressure–110°C. Add agar to distilled/deionized

water and bring volume to 800.0mL. Gently heat and

bring to boiling. Autoclave for 15 min at 15 psi pres

sure–121°C. To sterile agar solution, add 200.0mL of

sterile cherry filtrate. Distribute into sterile tubes.

Autoclave for 5 min at 15 psi pressure–121°C.

Use: For the cultivation of various fungi.