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Chopped Meat Carbohydrate Medium

[所属分类:培养基配方] [发布时间:2021-11-11] [发布人:网站管理员2] [阅读次数:] [返回]

Chopped Meat

Carbohydrate Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1240.0mL:

Peptone..............................................................30.0g

K2HPO4...............................................................5.0g

Yeast extract........................................................5.0g

Cellobiose ...........................................................1.0g

Maltose................................................................1.0g

Starch ..................................................................1.0g

L-Cysteine·HCl·H2O............................................0.5g

Chopped meat extract filtrate............................. 1.0L

Chopped meat extract solids....................... 200.0mL

Resazurin (0.025% solution)........................... 4.0mL

pH 7.0 ± 0.2 at 25°C

Chopped Meat Extract:

Composition per liter:

Beef or horse meat ..........................................500.0g

NaOH (1N solution)...................................... 25.0mL

Preparation of Chopped Meat Extract: Use

lean beef or horse meat. Remove fat and connective

tissue. Grind. Add meat and NaOH to distilled/deion

ized water and bring volume to 1.0L. Gently heat and

bring to boiling while stirring. Cool to 25°C. Remove

fat from surface. Filter. Reserve ground meat parti

cles and filtrate. Add distilled/deionized water to fil

trate and bring volume to 1.0L.

Preparation of Medium: To 1.0L of chopped

meat extract filtrate, add the remaining components,

except the L-cysteine·HCl·H2O and chopped meat

solids. Mix thoroughly. Gently heat to boiling. Cool

to room temperature. Add the L-cysteine·HCl·H2O.

Adjust pH to 7.0. Distribute 1 part chopped meat sol

ids (by volume) and 5 parts of liquid (by volume) into

tubes under O2-free 97% N2 + 3% H2. Cap with rub

ber stoppers and place tubes in a press. Autoclave for

15 min at 15 psi pressure–121°C with fast exhaust.

Use: For the cultivation of anaerobic bacteria, in

cluding Clostridium species.